Intermediate
Part:BBa_K1230014
Designed by: Andrés Ferriño Iriarte Group: iGEM13_Ciencias-UNAM (2013-09-26)
rbs-GFP-pLacI-rbs-MarA Generator
The MarA protein acts as a transcriptional activator in E. coli. It allows the expression of the acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to cationic antimicrobial peptides, such as LL-37. This composite part allows MarA expression in presence of IPTG, by using the LacI promoter and the RBS based on Elowitz repressilator.
This part has a GFP generator as a prefix.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1266
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 665
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Categories
Parameters
None |